Yeast 2 Hybrid (Y2H) system: protein protein interaction technique

What is Y2H, its requirements and how it works? An easy and animated video. Y2H is a molecular genetics technique to validate protein-protein interaction through downstream activation of a reporter gene. Yeast two-hybrid is based on reconstitution of a transcription factor (TF) fragments fused with two proteins whose interaction needs to be studied. Links: GFP: https://www.youtube.com/watch?v=4GszdFsd4jc&t=1s FRET: https://www.youtube.com/watch?v=qwMdtqgdap0 BiFC: https://www.youtube.com/watch?v=g6XeVH08xzY&t=1s by -Dr. Abhishek Bhandawat